Regulatory

Part:BBa_K564021:Design

Designed by: Sébastien Lemire   Group: iGEM11_DTU-Denmark   (2011-09-21)


aTC regulated promoter module: aTC--PoPS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 848
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 838
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

DNA was obtained by gene synthesis and a mutation within the -10 of the promoter driving expression of tetR makes it unsure whether it is produced or not by this part.


Source

DNA synthesis (integrated DNA technology)

References